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ORIGINAL ARTICLE
Year : 2012  |  Volume : 1  |  Issue : 2  |  Page : 79-84

An investigation of the relationshıp between coronary atherosclerosis and chromosome damage in human peripheral blood lymphocytes


1 Department of Biology, Faculty of Science, Istanbul University, Istanbul, Turkey
2 Department of Cardiology, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey

Correspondence Address:
Tuge Erkol
Department of Biology, Faculty of Science, Istanbul University, 34134, Vezneciler, Istanbul
Turkey
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2278-0521.100954

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Background: Coronary atherosclerosis is a disease of the coronary arteries and is a result of cumulative processes that are mostly seen in old-age population. Progressive accumulation of oxidative damage to cellular macromolecules, especially DNA, over a lifetime is one of the important causes of aging. This accumulation of damage may result in chromosome instability and this can be measured by micronucleus (MN) technique. Materials and Methods: In this study, MN technique was applied to observe the relationship between chromosome instability and proliferative effectiveness of lymphocytes and coronary atherosclerosis. Non-toxic levels of hydrogen peroxide were also administered to observe any existing differential effect due to this stress inducing oxidizing agent. Spontaneous and H 2 O 2 stress induced MN frequencies and proliferation state of peripheral lymphocytes were measured from both 30 patients diagnosed as coronary atherosclerosis and 30 healthy control individuals of the same age range. Results: There was no significant difference between spontaneous MN frequencies of patient and control groups (P = 0.08). Also, probability index (PI) values between these groups reflected no difference (P = 0.13). Hydrogen peroxide addition did not change spontaneous MN frequencies either in control (P = 0.23) or in patient group (P = 0.81). This non-toxic H 2 O 2 level failed to show any different effect on patients compared to controls (P = 0.41). Although increasing PI values were observed as a result of H 2 O 2 administration only in patient group, the comparisons between the two groups showed no significant difference (P = 0.34). Conclusion: Chromosome instability and proliferation index that were measured by MN technique in this study have no role in discriminating coronary atherosclerosis patients from healthy individuals.


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